Heterologous expression of staphylococcal enterotoxin B (seb) gene for antibody production
نویسندگان
چکیده
منابع مشابه
A Biosensor Method for a Competitive Immunoassay Detection of Staphylococcal Enterotoxin B (seb) in Milk2
A sensitive and more rapid biosensor method for the detection of staphylococcal enterotoxins (SE) is needed by the food industry. Staphylococcal enterotoxin B (SEB) is highly heat resistant and is a potential bioterrorism agent. Our research objective was to develop a competitive immunoassay using a surface plasmon resonance (SPR) biosensor for the detection of SEB below 1 ng/mL [part per billi...
متن کاملSequence Variability in Staphylococcal Enterotoxin Genes seb, sec, and sed
Ingestion of staphylococcal enterotoxins preformed by Staphylococcus aureus in food leads to staphylococcal food poisoning, the most prevalent foodborne intoxication worldwide. There are five major staphylococcal enterotoxins: SEA, SEB, SEC, SED, and SEE. While variants of these toxins have been described and were linked to specific hosts or levels or enterotoxin production, data on sequence va...
متن کاملSynthetic human monoclonal antibodies toward staphylococcal enterotoxin B (SEB) protective against toxic shock syndrome.
Staphylococcal enterotoxin B (SEB) is a potent toxin that can cause toxic shock syndrome and act as a lethal and incapacitating agent when used as a bioweapon. There are currently no vaccines or immunotherapeutics available against this toxin. Using phage display technology, human antigen-binding fragments (Fabs) were selected against SEB, and proteins were produced in Escherichia coli cells an...
متن کاملmolecular screening of staphylococcal enterotoxin b gene in clinical isolates
objective: the role of staphylococcal enterotoxin b (seb) in food poisoning is well known, however its role in other diseases remains to be explored. the aim of this study is the molecular screening and characterization of the seb gene in clinically isolated strains. materials and methods: in this experimentally study, 300 staphylococcus aureus (s. aureus) strains isolated from clinical samples...
متن کاملMurine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization.
A group of 14 monoclonal antibodies (mAbs) to staphylococcal enterotoxin B (SEB) were obtained by fusion of Sp2/O myeloma cells with spleen cells from female BALB/c mice immunized with commercial SEB. The antibodies belonged to IgG1 and IgG2b subclasses. We evaluated the anti-SEB titres, competition assays and sensitivity of detection by indirect ELISA. Reactivity and cross-reactivity were also...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2006
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol9-issue5-fulltext-8